FR3 Molecular Protocols

Filarial DNA Isolation - click here for Williams lab protocol for the 'BB' method of DNA isolation.

Filarial RNA Isolation - click here for Williams lab protocol for the 'BB' method of total RNA isolation.

Alternate RNA isolation protocol - developed in the Christensen lab for samples with high levels of blood contamination (i.e. microfilariae).

Filarial PCR and RT-PCR - click here for FR3 workshop exercise for amplifying the Tph-1 gene from DNA by PCR, and from total RNA by RT-PCR.

EST generation - click here for Filarial Genome Project protocols.

Molecular Diagnostics Protocols - click here for references to PCR and RT-PCR - based diagnostic tests for detection and differentiation of filarial parasites in blood and mosquitoes.