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L2 cDNA library (JHU96SL-BmL2)

Please quote reference number JHU96SL-BmL2 in the source section of your Genbank submission form when you submit sequence data from this library.

Vector: Uni-ZAP XR
Insert: Brugia malayi L2 cDNA.
Cloning Sites: Eco R I (5' end) and Xho I (3' end)
Unamplified Titer: 1 X 106 pfu/ml
Amplified Undiluted Titer: 2 X 109 pfu/ml
Average Insert Size: ~ 1000 bp
Background: < 4% non-recombinants
Host Strain: XL-1 Blue MRF' strain
Primers used in library constrcution:
5' end:
ESL: 5'
CCG GAA TTC CGG GGT TTA ATT ACC CAA GTT TGA G 3'
                          Eco RI                        spliced leader sequence
3' end:
XT: 5'
GCG CGC TCG AGC GTT TTT TTT TTT TTT TTT TTT 3'
                                Xho I

Host Strain Information:
The XL-1 Blue MRF' strain is the recommended host strain for amplification and screening of this Lambda Uni-ZAP cDNA library. The XL-1 Blue MRF' strain is restriction minus and is recommended for plating unamplified libraries, and for amplifying and screening libraries. It also enables blue/clear color selection of recombinant clones when grown on plates containing IPTG and X-gal.
The bacterial stock is stored and streaked on LB-tet plates and grown as overnight cultures in the absence of tetracycline.

Strain Genotype:

Δ (mcrA) 183, Δ (mcrCB-hsdSMR-mrr) 173, end A1, supE44, thi-1, recA1, gyrA96, relA1, lac [F' proAB, lac IqZΔ M15, Tn10 (tetr)].
Reference: Jerpseth, B., et al, (1992) Strategies 5: 81-83.

Upon Receiving the Library:
The library is frozen in 7% DMSO. Thaw in hand quickly and dilute 1:10 in SM buffer immediately. Add chloroform to 0.3%, mix, and store at 4oC.


Acknowledgements:
Nithyakalyani Raghavan and Alan Scott
Department of Molecular Microbiology and Immnology
School of Hygiene and Public Health
Johns Hopkins University
615 N. Wolfe St.
Baltimore, MD 21205

Tel: (410) 955-3442
FAX: (410) 955-0105