February

Protocols

SOP NUMBER	TITLE
8.2	Mosquito Rearing Techniques
8.3	Infecting Aedes aegypti with Brugia pahangi, B. malayi, and Dirofilaria immitis
8.4	Collecting Infective Larvae of Brugia pahangi, B. malayi, Dirofilaria immitis, and Dirofilaria repens From Infected Mosquitoes
8.5	Experimental Infection of Dogs, Cats, Ferrets, and Rodents with Dirofilaria immitis, Brugiamalayi, B. pahangi, or Dipetalonema reconditum
8.31	Standard Diet for Rearing Mosquitoes
8.56	Necropsy for Dirofilaria immitis in Dogs, Cats, and Ferrets
8.94	The Collection of Brugia sp. from Jirds, and the Surgical Transplantation of Adult Brugia sp. into Jirds
UWO1	Purification of Brugia mf by density gradient centrifugation
UWO2	Purification of Brugia mf by filtration
UWO3	Counting mf
SWDNA1	Smith DNA Isolation Protocol ('BB' method)
SWDNA2	Smith DNA Isolation Protocol (Trizol method)
SWRNA1	Smith RNA Isolation Protocol ('BB' method)
JFRNA2	RNA isolation protocol for samples with high level blood contamination
SWPCR1	Filarial PCR and RT-PCR (Tph-1 gene example)
SWEST1	Filarial Genome Project EST generation protocols
SWMD1	Molecular Diagnostics Protocol