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Adult male cDNA Library (JHU94SL-mWb)

Please quote reference number JHU94SL-mWb in the source section of your Genbank submission form when you submit sequence data from this library.

Vector: Uni-ZAP XR
Insert: Wucheriria bancrofti adult male cDNA.
Cloning Sites: Xba I (5' end) and EcoR I (3' end)
Unamplified Titer: 2.5 X 105 pfu/ml
Amplified Undiluted Titer: 1.2 X 109 pfu/ml
Average Insert Size: ~ 600 bp
Background: < 10% non-recombinants
Host Strain: XL-1 Blue MRF' strain
Primers used in library constrcution:
5' end:
XSL: 5'
GCT CTA GAG CGG TTT AAT TAC CCA AGT TTG AG 3'
                         Xba I                            spliced leader sequence
3' end:
XET: 5'
GCG CGC TCG AGA ATT TTT TTT TTT TTT TTT TTT 3'

Host Strain Information:
The XL-1 Blue MRF' strain is the recommended host strain for amplification and screening of this Lambda Uni-ZAP cDNA library. The XL-1 Blue MRF' strain is restriction minus and is recommended for plating unamplified libraries, and for amplifying and screening libraries. It also enables blue/clear color selection of recombinant clones when grown on plates containing IPTG and X-gal.
The bacterial stock is stored and streaked on LB-tet plates and grown as overnight cultures in the absence of tetracycline.

Strain Genotype:

Δ (mcrA) 183, Δ (mcrCB-hsdSMR-mrr) 173, end A1, supE44, thi-1, recA1, gyrA96, relA1, lac [F' proAB, lac IqZΔ M15, Tn10 (tetr)].
Reference: Jerpseth, B., et al, (1992) Strategies 5: 81-83.

Upon Receiving the Library:
The library is frozen in 7% DMSO. Thaw in hand quickly and dilute 1:10 in SM buffer immediately. Add chloroform to 0.3%, mix, and store at 4oC.


Acknowledgements:
Inca Ghosh, Nithyakalyani Raghavan, Ward Eisinger and Alan Scott
Department of Molecular Microbiology and Immnology
School of Hygiene and Public Health
Johns Hopkins University
615 N. Wolfe St.
Baltimore, MD 21205

Tel: (410) 955-3442
FAX: (410) 955-0105