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Genomic library (OvG)

Vector: Lambda FIX II (Xho I Cut- Stratagene)
Insert: Onchocerca volvulus Adult Female genomic DNA.
Cloning Site: Xho I cut vector ligated with Sau 3A-digested partially filled in insert
Unamplified Titer: 1.3 X 105 pfu
Amplified Undiluted Titer: ~ 2.3 x 106 pfu/ml
Insert Size Range 9-23 kb
Host Strain: XL-1 Blue MRA (P2) strain

Host Strain Information:
The XL-1 Blue MRA (P2) strain is the recommended host strain for amplification and screening of this Lambda FIX genomic library. The XL-1 Blue MRA (P2) strain is restriction minus and is recommended for plating unamplified libraries, and for amplifying and screening libraries. The bacterial stock is stored and streaked on LB plates and grown as overnight cultures.

Strain Genotype:
•(mcrA)183 •(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 gyrA96 relA1 lac P2 Lysogen

Upon Receiving the Library:
The library is frozen in 7% DMSO. Thaw in hand quickly and dilute 1:10 in SM buffer immediately. Add chloroform to 0.3%, mix, and store at 4oC.

Acknowledgements:

Michelle Lizotte-Waniewski and Dr. Steven A. Williams
Department of Biological Sciences
Clark Science Center
Smith College
Northampton, MA 01063

Tel: (413) 585-3826
FAX: (413) 585-3952