FR3 Molecular Resources
FR3 Next Generation Sequencing Service
Is an opportunity to collaborate with the FR3 scientists at Smith College on your research project, and to gain hands on experience with the Illumina MiSeq. For more detailed information about our sequencing service, click here.
Filarial RNA and DNA preparations
Filarial RNA and DNA preparations are now available through BEI Resources. If you are not currently registered with BEI, fill out the BEI Resources Material Transfer Agreement and Registration forms, then locate the FR3 catalog to order FR3 materials through BEI.
Species | Preparation | Life Cycle Stage(s) |
---|---|---|
Brugia malayi | total RNA | adult female adult male L3 L4 microfilariae |
B. pahangi | total RNA | adult female adult male microfilariae |
Dirofilaria immitis | total RNA | adult female adult male L3 microfilariae |
Aedes aegypti | total RNA | eggs male pupae young adult adult male adult female |
B. malayi | genomic DNA | ----- |
B. pahangi | genomic DNA | ---- |
D. immitis | DNA | ----- |
Acanthocheilonema viteae | DNA | ----- |
Aedes aegypti | DNA | ----- |
PCR primers available through BEI Resources
Primer Database
cDNA LIBRARIES - cDNA CLONES FROM INDICATED (*) LIBRARIES MUST BE ORDERED FROM BEI LABORATORIES (www.beiresources.org). OTHER cDNA LIBRARIES ARE ORDERED THROUGH FR3 MOLECULAR DIVISION.
Entire libraries can still be ordered through FR3 molecular division. Fill out the BEI Resources Material Transfer Agreement and Registration, then use the 'FR3 Ordering' link on the BEI homepage to get started.
GENOMIC LIBRARIES
B. malayi BAC library clones - used to map the Wolbachia genome (see Foster et al. 2004. Int. J. Parasit. 34: 733-746; and Foster et al. 2001. BioTechniques 30(6): 1216-1222). Please contact Smith College (genome@smith.edu) for ordering information.
FILARIAL SERUM SAMPLES/ANTIGENS
Bm14 Antigen for serologic monitoring
Positive Control for the Binax Filariasis Now test
BmWSP MONOCLONAL ANTIBODY - THIS MONOCLONAL IS AVAILABLE THROUGH BEI LABORATORIES (www.beiresources.org).
Real-time PCR Assay for the Simultaneous Detection of Wuchereria bancrofti and Brugia malayi
When conducted in accordance with the published protocol [1], this multiplex real-time PCR assay is capable of detecting Wuchereria bancrofti and Brugia malayi DNA simultaneously, or independently, at sensitivities which parallel the published singleplex assays for parasite detection. This assay has been tested on worm extracted DNA, as well as DNA extracted from both human bloodspots and vector mosquito pools. Xenomonitoring studies conducted in co-endemic locations can benefit from the reagent-cost and labor-cost related savings that this assay is able to provide.
1). Pilotte N, Torres M, Tomaino FR, Laney SJ, Williams SA. A TaqMan-based multiplex real-time PCR assay for the simultaneous detection of Wuchereria bancrofti and Brugia malayi. Mol Biochem Parasitol 2013 May; 189(1-2): 33-37.
Smith College will provide primers, controls and protocol for this procedure.
Please contact (genome@smith.edu) for further information regarding this assay.
FR3 Brugia malayi list of putative T cell epitopes
A list of putative T cell epitopes identified using the iVax Immunoinformatics Toolkit, by screening over 11,000 proteins belonging to the genome-derived B. malayi protein sequences (EpiVax, Providence, Rhode Island), in collaboration with Dr. Anne De Groot at the University of Rhonde Island.
Brugia malayi putative T cell epitopes