Research Materials Available from FR3 Molecular Resources

cDNA LIBRARIES

Characteristics of the Onchocerca volvulus Microfilaria cDNA Library. Please click each library to enter its data sheet.

*Onchocerca* *volvulus*	Microfilarial cDNA Library (SAW98MLW-OvMf) L2 cDNA Library (SAW98MLW-OvL2) Infective L3 cDNA Library (SAW94WL-OvL3) Adult female cDNA Library (SAW98MLW-OvAF) Adult male cDNA Library (SAW98MLW-OvAM) Adult female ivermectin treated cDNA Library (SAW99PF-OvAF) Genomic library (OvG)
Onchocerca ochengi	Infective L3 cDNA Library (SAW98MLW-OoL3)
Brugia malayi	Microfilarial cDNA library (SAW94LS-BmMf) L2 cDNA library (JHU96SL-BmL2) Infective L3 cDNA library (SAW94WL-BmL3) L3 molting-day 6 larval cDNA library (SAW96MLW-BmL3d6) L3 molting-day 9 larval cDNA library (SAW96MLW-BmL3d9) L3 subtracted cDNA library (SAW97LS-BmL3SA) L3 subtracted cDNA library (SAW97YG-BmL3SB) L3 subtracted cDNA library (SAW97LS-BmL3SD) L4 cDNA library (SAW99MLW--BmL4) Young adult cDNA library (SAW99MLW-BmYA) Adult male cDNA library (SAW94NL-BmAM) Adult female cDNA library (SAW96MLW-BmAF) Adult cDNA library (JHU96SL-BmA) Genomic library (BmG)
Wuchereria bancrofti	Microfilarial cDNA Library (SAW95SJL-WbMf) Infective L3 cDNA Library (SAW96MLW-WbL3) Adult female cDNA Library (JHU94SL-fWb) Adult male cDNA Library (JHU94SL-mWb)

Library Name	SAW98MLW-OvMf
Key:	SAW	Lab: SAW = Steven A. Williams' Lab JHU = Johns Hopkins University - Alan Scott's Lab
	98	year constructed
	MLW	initials of the individual who constructed the library
	Ov	species: Ov = Onchocerca volvulus Oo = Onchocerca ochengi Bm = Brugia malayi Wb = Wuchereria bancrofti
	Mf	stage: Mf = microfilaria L2 = second larval stage L3 = third larval stage L3d6 = L3 to L4 molt, day 6 L3d9 = L3 to L4 molt, day 9 L3SA (L3SD) = L3 cDNA subtracted with microfilaria, adult male and adult female cDNA L3SB = L3 cDNA obtained by subtracting JHU93SL-BmL3 cDNA library (constructed using SL sequence and PCR) from SAW94WL-BmL3 cDNA library (constructed using conventional cDNA methods). L4 = fourth larval stage YA = young adult AF = adult female AM = adult male

B. malayi MICROARRAY

The second version (V.2) of the filarial microarrays are now available through the Molecular Division of FR3. These can be requested just like any other reagent using the Requisition Form found on this site. The only additional requirement is that the individual lab requesting the arrays must summarize briefly the experiment for which the arrays are being requested. Please see the MIAME protocol and checklist. This is so I can inform the lab requesting the arrays if another lab is already doing a substantially similar experiment. This will not prevent any lab from doing any experiment they wish, it is merely to keep everyone informed of any duplication of effort. This may enable laboratories to modify their plans in order to ask slightly different questions.

The arrays are made available through the efforts and funding of the Filarial Microarray Consortium (FMC). The FMC includes the following individuals who contributed funds for the design and printing of the arrays: Steven A. Williams (Smith College), Gary Weil (Washington University), David Spiro (TIGR), Barton Slatko (New England Biolabs), Thomas Nutman (NIAID), Thomas Klei (LSU), Achim Hoerauf (University of Bonn), Sara Lustigman (New York Blood Center), Brenda Beerntsen (U. Missouri), TV Rajan (U. Connecticut Health Center), and Alan Scott (Johns Hopkins). Special thanks to David Spiro and Seth Crosby ( Washington University) for their hard work in much of the design of the arrays.

The 18,153 Oligonucleotides on the Version 2 (V.2) array include:

9,241 oligos based on the TIGR B. malayi gene models (predicted genes)
1,889 oligos based on TIGR gene indices (EST clusters and singletons not represented in the gene models above)
81 oligos based on NEMBASE EST clusters (those not already represented above)
675 oligos based on EST clusters from L3 EST clusters from Wash. U. Parasite Genomes (those not already represented above)
878 oligos based on EST clustering of W. bancrofti data (those not already represented above)
1,016 oligos based TIGR O. volvulus gene indices (EST clusters and singletons not already represented above)
804 oligos based on the search of the Wolbachia complete genome for open reading frames
3,569 oligos based on the original B. malayi EST clusters from the version 1 array (McPherson oligos)

The B. malayi microarray platform has been extensively validated by quantitative reverse transcriptase polymerase chain reaction (Li et al. 2005. Mol. Biochem. Parasitol. 143:49-57) and tissue localization by in situ hybridization (Jiang et al. 2008. Int. J. Parasitol. 38:503-12). For further information, consult the Filarial Genomics and Bioinformatics page, or contact Steven A. Williams, Director, Molecular Division FR3 at genome@smith.edu.

GENOMIC LIBRARIES

B. malayi BAC library clones - used to map the Wolbachia genome (see Foster et al. 2004. Int. J. Parasit. 34: 733-746; and Foster et al. 2001. BioTechniques 30(6): 1216-1222).

FILARIAL SERUM SAMPLES/ANTIGENS

Bm14 Antigen for serologic monitoring

Positive Control for the Binax Filariasis Now test

Filarial Serum Bank

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