FR3 Molecular Resources
Molecular Resources List
Filarial RNA and DNA preparations
Filarial RNA and DNA preparations are now available through BEI Resources. If you are not currently registered with BEI, fill out the BEI Resources Material Transfer Agreement and Registration forms, then use the FR3 ordering link on the BEI homepage to get started.
|Species||Preparation||Life Cycle Stage(s)|
|Brugia malayi||total RNA||adult female
|B. pahangi||total RNA||adult female
|Dirofilaria immitis||total RNA||adult female
|Aedes aegypti||total RNA||eggs
|B. malayi||genomic DNA||-----|
|B. pahangi||genomic DNA||----|
PCR primers available through BEI Resources
cDNA LIBRARIES - cDNA CLONES FROM INDICATED (*) LIBRARIES MUST BE ORDERED FROM BEI LABORATORIES (www.beiresources.org).
Entire libraries can still be ordered through FR3. Fill out the BEI Resources Material Transfer Agreement and Registration, then use the 'FR3 Ordering' link on the BEI homepage to get started.
B. malayi BAC library clones - used to map the Wolbachia genome (see Foster et al. 2004. Int. J. Parasit. 34: 733-746; and Foster et al. 2001. BioTechniques 30(6): 1216-1222). Please contact Smith College (email@example.com) for ordering information.
FILARIAL SERUM SAMPLES/ANTIGENS
BmWSP MONOCLONAL ANTIBODY - THIS MONOCLONAL IS AVAILABLE THROUGH BEI LABORATORIES (www.beiresources.org).
Real-time PCR Assay for the Simultaneous Detection of Wuchereria bancrofti and Brugia malayi
When conducted in accordance with the published protocol , this multiplex real-time PCR assay is capable of detecting Wuchereria bancrofti and Brugia malayi DNA simultaneously, or independently, at sensitivities which parallel the published singleplex assays for parasite detection. This assay has been tested on worm extracted DNA, as well as DNA extracted from both human bloodspots and vector mosquito pools. Xenomonitoring studies conducted in co-endemic locations can benefit from the reagent-cost and labor-cost related savings that this assay is able to provide.
1). Pilotte N, Torres M, Tomaino FR, Laney SJ, Williams SA. A TaqMan-based multiplex real-time PCR assay for the simultaneous detection of Wuchereria bancrofti and Brugia malayi. Mol Biochem Parasitol 2013 May; 189(1-2): 33-37.
Smith College will provide primers, controls and protocol for this procedure.
Please contact (firstname.lastname@example.org) for further information regarding this assay.
FR3 Brugia malayi list of putative T cell epitopes
A list of putative T cell epitopes identified using the iVax Immunoinformatics Toolkit, by screening over 11,000 proteins belonging to the genome-derived B. malayi protein sequences (EpiVax, Providence, Rhode Island), in collaboration with Dr. Anne De Groot at the University of Rhonde Island.