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Molecular Resources List

Filarial RNA and DNA preparations

Filarial RNA and DNA preparations are now available through BEI Resources.  If you are not currently registered with BEI, fill out the BEI Resources Material Transfer Agreement and Registration forms, then use the FR3 ordering link on the BEI homepage to get started.

Species Preparation  Life Cycle Stage(s)
Brugia malayi total RNA  adult female
adult male
L3
L4
microfilariae
B. pahangi total RNA adult female
adult male
microfilariae
Dirofilaria immitis total RNA adult female
adult male
L3 
microfilariae
Aedes aegypti total RNA eggs
male pupae
young adult
adult male
adult female
B. malayi genomic DNA  ----- 
B. pahangi genomic DNA   ----
D. immitis DNA  ----- 
Acanthocheilonema viteae DNA  ----- 
Aedes aegypti DNA  ----- 

 

PCR primers available through BEI Resources

Filarial PCR primers are now available through BEI Resources.  If you are not currently registered with BEI, fill out the BEI Resources Material Transfer Agreement and Registration forms, then use the FR3 ordering link on the BEI homepage to get started.

primer_list_2014

 

cDNA LIBRARIES - cDNA CLONES FROM INDICATED (*) LIBRARIES MUST BE ORDERED FROM BEI LABORATORIES (www.beiresources.org).

Entire libraries can still be ordered through FR3.  Fill out the BEI Resources Material Transfer Agreement and Registration, then use the 'FR3 Ordering' link on the BEI homepage to get started.

Characteristics of the Onchocerca volvulus Microfilaria cDNA Library.  Please click each library to enter its data sheet.
 
Onchocerca volvulus  Microfilarial cDNA Library (SAW98MLW-OvMf)*
 L2 cDNA Library (SAW98MLW-OvL2)*
 Infective L3 cDNA Library (SAW94WL-OvL3)*
 Adult female cDNA Library (SAW98MLW-OvAF)*
 Adult male cDNA Library (SAW98MLW-OvAM)*
 Adult female ivermectin treated cDNA Library (SAW99PF-OvAF)
 Genomic library (OvG)
Molting L3 cDNA Library (Ov3M)*
Onchocerca ochengi  Infective L3 cDNA Library (SAW98MLW-OoL3)
Brugia malayi  Microfilarial cDNA library (SAW94LS-BmMf)
 L2 cDNA library (JHU96SL-BmL2)
 Infective L3 cDNA library (SAW94WL-BmL3)
 L3 molting-day 6 larval cDNA library  (SAW96MLW-BmL3d6)
 L3 molting-day 9 larval cDNA library (SAW96MLW-BmL3d9)
 L3 subtracted cDNA library (SAW97LS-BmL3SA)
 L3 subtracted cDNA library (SAW97YG-BmL3SB)*
 L3 subtracted cDNA library (SAW97LS-BmL3SD)*
 L4 cDNA library (SAW99MLW--BmL4)*
 Young adult cDNA library (SAW99MLW-BmYA)*
 Adult male cDNA library (SAW94NL-BmAM)*
 Adult female cDNA library (SAW96MLW-BmAF)*
 Adult cDNA library (JHU96SL-BmA)
 Genomic library (BmG)
Wuchereria bancrofti  Microfilarial cDNA Library (SAW95SJL-WbMf)*
 Infective L3 cDNA Library (SAW96MLW-WbL3)
 Adult female cDNA Library (JHU94SL-fWb)
 Adult male cDNA Library (JHU94SL-mWb)

 
Library Name   SAW98MLW-OvMf
 Key: SAW Lab:
SAW = Steven A. Williams' Lab
JHU = Johns Hopkins University - Alan Scott's Lab
  98 year constructed
  MLW initials of the individual who constructed the library
  Ov species:
Ov = Onchocerca volvulus
Oo = Onchocerca ochengi
Bm = Brugia malayi
Wb = Wuchereria bancrofti
  Mf stage:
Mf = microfilaria
L2 = second larval stage
L3 = third larval stage
L3d6 = L3 to L4 molt, day 6
L3d9 = L3 to L4 molt, day 9
L3SA (L3SD) = L3 cDNA subtracted with microfilaria, adult male and
adult female cDNA
L3SB = L3 cDNA obtained by subtracting JHU93SL-BmL3 cDNA library (constructed using SL sequence and PCR) from SAW94WL-BmL3 cDNA library (constructed using conventional cDNA methods).
L4 = fourth larval stage
YA = young adult
AF = adult female
AM = adult male

 

 

GENOMIC LIBRARIES

B. malayi BAC library clones - used to map the Wolbachia genome (see Foster et al. 2004. Int. J. Parasit. 34: 733-746; and Foster et al. 2001BioTechniques 30(6): 1216-1222).  Please contact Smith College (genome@smith.edu) for ordering information.

 

FILARIAL SERUM SAMPLES/ANTIGENS

TO ORDER FILARIAL SERUM SAMPLES AND ANTIGENS, YOU MUST HAVE A CURRENT FR3 REGISTRATION FORM ON FILE, AND A SMITH COLLEGE MTA. 
 

Bm14 Antigen for serologic monitoring

Positive Control for the Binax Filariasis Now test

Filarial Serum Bank

 

BmWSP MONOCLONAL ANTIBODY - THIS MONOCLONAL IS AVAILABLE THROUGH BEI LABORATORIES (www.beiresources.org). 

Fill out the BEI Resources Material Transfer Agreement and Registration, then use the FR3 Ordering link on the BEI homepage to get started.  This monoclonal was developed by Patrick Lammie at Centers for Disease Control, and recognizes wBm0432.

Real-time PCR Assay for the Simultaneous Detection of Wuchereria bancrofti and Brugia malayi

 When conducted in accordance with the published protocol [1], this multiplex real-time PCR assay is capable of detecting Wuchereria bancrofti and Brugia malayi DNA simultaneously, or independently, at sensitivities which parallel the published singleplex assays for parasite detection.  This assay has been tested on worm extracted DNA, as well as DNA extracted from both human bloodspots and vector mosquito pools.  Xenomonitoring studies conducted in co-endemic locations can benefit from the reagent-cost and labor-cost related savings that this assay is able to provide.

1).  Pilotte N, Torres M, Tomaino FR, Laney SJ, Williams SA.  A TaqMan-based multiplex real-time PCR assay for the simultaneous detection of Wuchereria bancrofti and Brugia malayi.  Mol Biochem Parasitol 2013 May; 189(1-2): 33-37. 

Smith College will provide primers, controls and protocol for this procedure.  

Please contact  (genome@smith.edu) for further information regarding this assay.  

 

FR3 Brugia malayi list of putative T cell epitopes

A list of putative T cell epitopes identified using the iVax Immunoinformatics Toolkit, by screening over 11,000 proteins belonging to the genome-derived B. malayi protein sequences (EpiVax, Providence, Rhode Island), in collaboration with Dr. Anne De Groot at the University of Rhonde Island. 

Brugia malayi putative T cell epitopes

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