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L2 cDNA Library (SAW98MLW-OvL2)

Please quote reference number SAW98MLW-OvL2 in the source section of your Genbank submission form when you submit sequence data from this library.

Vector: Lambda Uni-ZAP XR (Lambda ZAP cut with Eco RI and Xho I - Stratagene)
Insert: Onchocerca volvulus L2 cDNA.
Cloning Sites: EcoRI (5' end) and XhoI (3' end)
Unamplified Titer: 7.3 X 104 pfu
Amplified Undiluted Titer: ~ 1010 pfu/ml
Average Insert Size: ~ 1030 bp
Background: 30% non-recombinants
Host Strain: XL-1 Blue MRF' strain

Host Strain Information:
The XL-1 Blue MRF' strain is the recommended host strain for amplification and screening of this Lambda Uni-ZAP cDNA library. The XL-1 Blue MRF' strain is restriction minus and is recommended for plating unamplified libraries, and for amplifying and screening libraries. It also enables blue/clear color selection of recombinant clones when grown on plates containing IPTG and X-gal.
The bacterial stock is stored and streaked on LB-tet plates and grown as overnight cultures in the absence of tetracycline.

Strain Genotype:
Δ (mcrA) 183, Δ (mcrCB-hsdSMR-mrr) 173, end A1, supE44, thi-1, recA1, gyrA96, relA1, lac [F' proAB, lac IqZΔ M15, Tn10 (tetr)].
Reference: Jerpseth, B., et al, (1992) Strategies 5: 81-83.

Upon Receiving the Library:
The library is frozen in 7% DMSO. Thaw in hand quickly and dilute 1:10 in SM buffer immediately. Add chloroform to 0.3%, mix, and store at 4oC.

Michelle Lizotte-Waniewski and Steven A. Williams
Department of Biological Sciences
Clark Science Center
Smith College
Northampton, MA 01063

Tel: (413) 585-3826
FAX: (413) 585-3786